Biomedical Engineering Reference
In-Depth Information
Table 11.3: Binding rate coefficients and fractal dimensions for (a) bulk concentration of free DNA in solution (1 nM) to
sensor surface, (b) nonmatching (non complementary; m22; 5
0
-Cy3-TGA-GCG-TTC-GTG-GTG-GGA-TAG-T-3
0
), and
matching sequence (complementary; p22; 5
0
-Cy3-ACT-CGC-AAG-CAC-CAC-CCT-ATC-A-3
0
) in solution to one strand
(bound DNA; i22; 5
0
-NH
2
-C6-TTT-TTT-TTT-TTT-TTT-TGA-TAG-GGT-GGT-GCT-GGT-GCT-TGC-GAG-T-3
0
) immobilized
on a glass substrate (
Michel et al., 2007
).
Strand in Solution/
Receptor on
Surface
k
k
1
k
2
D
f
D
f1
D
f2
1 nM free DNA/
surface
0.006628 0.000415
na
na
2.3966 0.02612
na
na
5.7 10
05
3 10
05
Noncomplementary
(m22)/i22
na
na
1.7036 0.1151
na
na
Complementary
(p22)/i22
0.06240
0.01266
0.008629
0.000020 0.4462
0.0162 2.2074
0.1243
1.4592
0.005772
2.7794
0.05058
