Biomedical Engineering Reference
In-Depth Information
0.8
0.6
0.5
0.6
0.4
0.4
0.3
0.2
0.2
0.1
0
0
0
500
1000
1500
2000
0
500
1000
1500
2000
B
Time (s)
A
Time (s)
0.35
0.5
0.3
0.4
0.25
0.3
0.2
0.15
0.2
0.1
0.1
0.05
0
0
0
500
1000
1500
2000
0
500
1000
1500
2000
C
D
Time (s)
Time (s)
0.25
0.2
0.15
0.1
0.05
0
0
500
1000
1500
2000
E
Time (s)
Figure 9.1
Binding and dissociation of different bradykinin concentrations (in nM) to bradykinin B
2
receptors
immobilized on a resonant waveguide grating (RWG) biosensor (Fang et al., 2006): (a) 128
(b) 64 (c) 32 (d) 16 (e) 8. When only a solid line (--) is used then a single-fractal analysis applies.
When both a dashed (- - -) and a solid (--) line are used then the dashed line represents a
single-fractal analysis and the solid line represents a dual-fractal analysis.
dual-fractal analysis is required to adequately describe the binding and the dissociation kinetics.
The values of (a) the binding rate coefficient,
k
, and the fractal dimension,
D
f
, for a single-fractal
analysis, (b) the binding rate coefficients,
k
1
and
k
2
, and the fractal dimensions,
D
f1
and
D
f2
,
for a dual-fractal analysis, and (c) the dissociation rate coefficient,
k
d
, and the fractal dimension,
D
fd
, for a single-fractal analysis, and (d) the dissociation rate coefficients,
k
d1
and
k
d2
, and the
fractal dimensions,
D
fd1
and
D
fd2
, for a dual-fractal analysis are given in
Tables 9.1
and
9.2
.
