Design and Development of In Vivo Sensor Systems: The Long and Tortured Road to a Self-Contained, Implantable Glucose Sensor for Diabetes Management - Designing Receptors for the Next Generation of Biosensors - page 209

4 Glucose-Competitive DBA Binding Environment (iDIOL) . . ............................ 224
4.1 iDIOLs as Competitive Binding Environments for Glucose Detection ............ 225
5 Coordinated Identification of DBA:iDIOL Lead Pairs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 226
6 Glucose Competition Binding Assay ...................................................... 228
6.1 Examples of Different Component Sensitivity and Selectivity ..................... 229
6.2 Implications of Binding Affinity Studies on Component Pair Selection . . ......... 233
7 Concluding Remarks . . . . ................................................................... 233
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 234

Abbreviations

ARS Alizarin Red S; also known as 3,4-dihydroxy-9,10-dioxo-2-anthracene-

sulfonic acid sodium salt

DBA Dendrimer-boronic acid

IC 50 Half Maximal Inhibitory Concentration

iDIOL Immobilized diol/saccharide analogue

Keq Equilibrium constant

MIP Moleculary imprinted polymer

NIR Near-infrared

PET Photoinduced electron transfer

p K a The negative logarithm of the dissociation constant

RFID Radio frequency identification

Definitions

DBA A dendrimer construct that is functionalized with boronic
acid receptor ligands and a fluorescent reporter moiety. The
DBA is the sensing system signaling component that can
competitively bind to the glucose analyte and to the 1,2-
and 1,3-dihydroxy motif(s) of iDIOLs.
diol A saccharide analogue moiety that typically contains a 1,2-
or 1,3-dihydroxy motif and a functional group that can be
used for covalent immobilization of the diol on a support to
create an iDIOL.
iDIOL An immobilized diol/saccharide analogue that contains a
1,2- or 1,3-dihydroxy moiety that is covalently attached to
a support. The iDIOL competes with glucose for DBA
binding, which produces a bound versus free sensing sys-
tem signal.
DBA:glucose:iDIOL Designation of the three component competitive system
where glucose and the iDIOL compete for DBA binding
to produce a signal response that is proportional to glucose
concentration.