Design and Development of In Vivo Sensor Systems: The Long and Tortured Road to a Self-Contained, Implantable Glucose Sensor for Diabetes Management - Designing Receptors for the Next Generation of Biosensors - page 208

Design and Development of In Vivo Sensor
Systems: The Long and Tortured Road
to a Self-Contained, Implantable Glucose
Sensor for Diabetes Management
Christina Thomas, Rachel Weller Roska, and Robert E. Carlson
Abstract We have successfully completed the development of a glucose sensing
system, which is the mission-critical component of an implantable glucose sensor
for use by diabetic patients. This proof-of-principle demonstration showed that the
closed-cycle, self-contained glucose sensing system can produce a consistent,
measurable response to physiologically relevant levels of glucose while functioning
under biologically relevant conditions. The sensing system requires the interaction
of two components: (1) a competitive agent/signaling component, which is a
dendrimer-boronic acid (DBA) construct and (2) a glucose-competitive DBA binding
environment, which is an immobilized monosaccharide mimic (iDIOL). The
demonstrated sensing system meets our primary stability, sensitivity, and specific-
ity criteria. These results, accompanied by our library of synthetic materials and
binding affinity database, provide a firm foundation upon which to optimize the
glucose sensing system and incorporate it into the implantable sensor device.
Keywords Artificial receptor, Binding affinity, Boronic acid, Closed-cycle,
Competitive binding assay, Continuous, Dendrimer-boronic acid, Glucose sensing
system, Glucose-competitive binding environment, Implantable, Noninvasive,
Saccharide
Contents
1 Diabetes and Blood Glucose Management . . .............................................. 215
2 Artificial Receptor Technology and the Development of a Sensing System for an
Implantable Glucose Sensor . ............................................................... 217
3 Self-Contained and Closed-Cycle Stable Glucose Sensing System ...................... 219
3.1 Competition/Signaling Component (DBA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 220
C. Thomas, R.W. Roska, and R.E. Carlson ( * )
RECEPTORS LLC, Suite 510/MD 57, 1107 Hazeltine Boulevard, Chaska, MN 55318-2533, USA
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