Biomedical Engineering Reference
In-Depth Information
C
O
Au
Ag
Au
012345
6789 0
Energy/Kev
FIGURE 14.8
In EDX analysis of Ag
-loaded tissue conditioner, the spectrum shows the characteristic peak for Ag at 3 Kev.
Unassigned peaks originate from polymer or external contaminants.
sputtered under high vacuum before the analysis. SEM image of 2.0% Ag
-tissue conditioner
(
Figure 14.7A
) exhibited similar surface texture some enlarged porosities as compared to control
(
Figure 14.7B)
. Energy dispersive X-ray (EDX) studies were also performed for Ag
-tissue condi-
tioner to identify the Ag
in the composites. EDX analysis identified Ag peak clearly which shows
that Ag
were successfully immobilized into the Ag
-tissue conditioner (
Figure 14.8
).
14.5
Conclusions
Experimental acrylic tissue conditioner containing silver nanoparticles (Ag
-tissue conditioner) was
characterized in regards to its antimicrobial effect and cytocompatibility. Formations of silver nano-
particles (50
80 nm) were evaluated by TEM, UV-vis spectroscopy (409.7 nm), and the identifica-
tion of Ag
in the compound was confirmed by EDX analysis. The prepared Ag
-tissue conditioner
has the great bactericidal/fungicidal activity with reduction of 99.9% of S. aureus, S. mutans at
0.1% of Ag
incorporated, and C. albicans at 0.5% of Ag
combined over the control (0% of Ag
)
value. Up to 0.5% Ag
showed no cytotoxic influence on HGF. Thus, this chapter concludes that
within the limitations of the present in vitro study, Ag
-loaded acrylic tissue conditioner could be a
possible
candidate
for
a
novel
antimicrobial
tissue
conditioner with
uncompromised
cytocompatibility.
