Biomedical Engineering Reference
In-Depth Information
TABLE 17.2
Total.Coliform.(TC).cfu/ml.of.Wastewater.Samples.before.and.after.Treatment.with.MMN
TimeofIncubationof
Wastewater
Sample
with
MMN
TCinOriginal
Sample
a
TCinSupernatant
b
TCinPellet
b
10.min
1.2.*.10
8.
cfu/ml
1.1.*.10
5
.cfu/ml
1.6.*.10
5
.cfu/ml
20.min
1.2.*.10
8.
cfu/ml
4.0.*.10
4
.cfu/ml
4.4*.10
5.
cfu/ml
30.min
1.2.*.10
8.
cfu/ml
7.0.*.10
4
.cfu/ml
1.4.*.10
5
.cfu/ml
40.min
1.2.*.10
8.
cfu/ml
9.0.*.10
4
.cfu/ml
3.6.*.10
5
.cfu/ml
a.
Before.treatment.with.MMN.
b.
After.treatment.with.MMN.
tubes.for.20.min..The.supernatant.was.removed.(while.the.magnet.was.still.on).
and.transferred.into.another.sterile.tube,.and.the.aggregated.pellet.was.washed.
with. sterile. deinoized. water.. For. every. sample. both. the. supernatant. and. aggre-
gated.pellet.were.examined.under.a.TEM.
•. Serial.dilutions.for.each.tube,.including.the.supernatant.and.pellet,.were.done;.0.1.
ml.from.each.of.the.different.dilutions.was.inoculated.in.nutrient.agar.and.EMB.
agar. plates. and. incubated. for. 24. h. at. 37°C.. Then. the. total. heterotrophic. aerobic.
bacterial.counts.and.total.coliform.counts.(cfu/ml).were.recorded.
17.2.9 Treatment of Wastewater: Using MMNs for Detection,
Enumeration, and Removal of Bacterial Contamination
Wastewater.samples.were.collected.in.sterile.containers.from.different.wastewater.treatment.
plants.in.Jordan..The.samples.were.brought.on.ice.to.the.laboratory.for.examination.
•. Five.milliliters.of.each.wastewater.sample.was.placed.in.a.sterile.tube.
•. Serial.dilutions.were.done,.and.0.1.ml.from.each.dilution.was.inoculated.into.nutri-
ent.agar.medium.plates.and.0.1.ml.onto.an.EMB.agar.plate.medium.and.incubated.
for.24.h.at.37°C..The.total.heterotrophic.bacterial.and.coliform.counts.(cfu/ml).on.
each.type.of.media.were.recorded.
•. The. MMNs. preparation. (200. mg). was. suspended. in. 50. ml. of. sterile. deionoized.
water.(4 mg/ml),.and.200.μl.of.this.MMN.suspension.was.added.to.the.wastewater.
tube..Four.replicates.of.the.previous.mixtures.were.used.at.different.incubation.
times.(10,.20,.30,.and.40.min).to.evaluate.the.sensitivity.of.time.in.this.assay..These.
four.replicates.of.mixtures.were.incubated.with.shaking.at.37°C.
•. A.magnet.(400.G).was.placed.on.the.side.wall.of.each.of.the.four.tubes.for.10-20.
min,.the.supernatant.was.removed.(while.the.magnet.was.still.on).and.transferred.
into. another. sterile. tube,. and. the. pellet. was. washed. by. sterile. deionoized. water..
Both.the.supernatant.and.pellet.were.examined.under.a.TEM.(Figure 17.5a.and.b).
•. Serial.dilutions.for.each.tube.(for.both.the.supernatant.and.the.pellet).were.done;.
0.1.ml.from.each.dilution.was.inoculated.in.nutrient.agar.and.EMB.agar.plates.and.
incubated. for. 24. h. at. 37°C.. Then. the. total. heterotrophic. aerobic. bacterial. counts.
and.the.total.coliform.counts.(cfu/ml).were.recorded.
