Biomedical Engineering Reference
In-Depth Information
(a)
(b)
.
FIGURE 17.5
(a). TEM. pictures. of. wastewater. sample. before. treatment. with. MMN.. (b). Wastewater. sample. after. treatment.
with.MMNs.and.use.of.magnet.
17.2.10 Use of MMNs for Detection, Enumeration, and Removal
of Bacterial Contamination in Grey Water
Grey.water.samples.were.brought.on.ice.in.sterile.containers.to.the.laboratory.
•. Five.milliliters.of.grey.water.was.placed.in.a.sterile.tube.
•. Serial. dilutions. were. done,. and. 0.1. ml. from. each. dilution. was. inoculated. into.
nutrient.medium.agar.plates.and.EMB.medium.agar.plates.and.incubated.for.24.h.
at.37°C..Then.the.total.heterotrophic.bacterial.and.coliform.counts.(cfu/ml).were.
recorded.(Table 17.3).
TABLE 17.3
Total.Coliform.(TC).cfu/ml.of.Grey.Water.Samples.before.and.after.Treatment.with.MMN
TimeofIncubationofGrey
WaterSamplewithMMN
TCinOriginal
Sample
a
TCinSupernatant
b
TCinPellet
b
10.min
1.2.*.10
7.
cfu/ml
1.0.*.10
4
.cfu/ml
1.8.*.10
5
.cfu/ml
20.min
1.2.*.10
7.
cfu/ml
3.1.*.10
4
.cfu/ml
4.0*.10
5.
cfu/ml
30.min
1.2.*.10
7.
cfu/ml
5.1.*.10
4
.cfu/ml
2.2.*.10
5
.cfu/ml
40.min
1.2.*.10
7.
cfu/ml
7.3.*.10
4
.cfu/ml
2.5.*.10
5
.cfu/ml
a.
Before.treatment.with.MMN.
b.
After.treatment.with.MMN.
