Sample Processing - Waterborne Pathogens: Detection Methods and Applications - page 73

Table 4.1 Some examples of recovery rates in percent ± SD for different filtration
setup and elution conditions for various microorganisms

Filtration
technique

Elution
technique

Water type/
volume

Surrogate
viruses 1 Filter Elution buffer Seeding level 1

Rexeed
25-S

Dead-end Back-flush 0.01% NaPP + 0.5%

Tween80 + 0.001%
Y-30 Antifoam

MS2: 0.39-1.0 ×
10 5 pfu
E. faecalis 0.9-
1.0 × 10 3 cfu
C. perfringens :
1.5-3.76 × 10 3
endospores
C. parvum : 0.9-
1.4 × 10 6 oocysts

Mix 2 (0.29
NTU)/
100 L

MS2:
57 ± 7.7

Mix 2 (1.5
NTU)/
100 L

MS2:
82 ± 14

Mix 2 (4.3
NTU)/
100 L

MS2:
73 ± 13

Fresenius
F80A

Tangential Back-

flushing

0.01% NaPP + 0.01%
Tween80 + 0.01%
Tween20
0.001% Antifoam A

All 10 6 pfu per
cfu per oocysts
per endospores

Tap/10 L MS2:

65 ± 35

0.01% NaPP +
0.5-1%Tween80 +
0.001% Antifoam A

MS2:
91 ± 33

Fresenius
F200NR

Tangential Back-

flushing

0.01% NaPP + 0.5%
Tween80 + 0.001%
Antifoam A

All 10 6 pfu per
cfu per oocysts
per endospores

Tap/100 L MS2:

84 ± 12

Elution 0.01% NaPP + 0.1%

Tween80 + 0.001%
Antifoam A

MS2:
53 ± 13

Fresenius
F200NR

Tangential Direct

collection

MS2: 6300 ±
3400 pfu
C. perfringens :
2100 ± 770
endospores
E. coli: 24,000 ±
16,000 cfu
C. parvum :
150,000 ± 52,000
oocysts

Reclaimed/
10 L

MS2:
110 ± 18

Elution 0.01% NaPP + 0.01%

Tween80 + 0.001%
Antifoam Y-30

MS2:
120 ± 20

Exeltra plus
210
(blocked
with
0.1%
NaPP)

Tangential Elution 0.01% NaPP + 0.1%

Tween80 + 0.001%
Antifoam A;
(Re-concentration:
Centricon Plus-70)

High; low (1-10
pfu or cfu per
100 mL −1 )

Surface/
100 L

MS2: 48
± 25; 84
PRD1: 43
± 32; ND
MNV:
ND; 74

Drinking/
100 L

MS2:
58 ± 10,
38 ± 12
PRD1: 80;
57 ± 16
MNV: 41
± 34, ND