Molecular Methods for the Detection of Waterborne Pathogens - Waterborne Pathogens: Detection Methods and Applications - page 253

Table 8.1 Major groups of waterborne microbial pathogens with their traditional and proposed detection methods—cont'd
Group Pathogen Traditional method Suggested new methods

Protozoa Acanthamoeba

castellanii

Concentration using depth filters fol-
lowed by microscopic examination
of slides

DNA microarray 67

Cryptosporidium
spp.

Concentration using depth filters fol-
lowed by oocyst detection by immu-
nofluorescent antibody (IFA) and
conformation by DIC microscopy:
USEPA method 1623 146

Nested PCR-RFLP 147-149
Multiplex PCR with Luminex assay 150
Microarray 67,73
LAMP 80
Microfluidics and biosensors 151

Nested PCR 152
PCR-OLA 153

Cyclospora spp. Concentration using depth filters fol-

lowed by microscopic examination
of slides

Entamoeba histo-
lytica

Concentration using depth filters fol-
lowed by microscopic examination
of slides

Microarray 73

PCR using SSU-rRNA 68 Microsporidium

( Enterocytozoon
spp., Encephali-
tozoon spp.)

Concentration using depth filters fol-
lowed by microscopic examination of
slides using Weber's stain 154