Biology Reference
In-Depth Information
Bacteria
Campylobacter
jejuni
Based on detection of fecal coliform
indicator bacteria on microbiologi-
cal media followed by species-specific
metabolic tests
Microaerophilic enrichment at 42 °C followed by PCR
of 16S rRNA
49
qPCR of
VS1
gene
41
Escherichia coli
O157:H7
Based on detection of fecal coliform
indicator bacteria on microbiological
media, confirmation with MUG
Microarray
51
MB-real-time PCR down to 10 cfu mL
−1
after 18 h of
enrichment
94,95
qPCR using gene detected 10 cells mL
−1
in wastewater
samples
41
FRET
97
MB microarray using FRET
92
Magnetic bead-quantum dot assay, could detect
25 cfu mL
−1
139
Legionella pneu-
mophila
Based on detection of fecal coliform indi-
cator bacteria on microbiological media
Microarray
86
qPCR combined with propidium monoazide (PMA)
140
Salmonella enterica
Based on detection of fecal coliform
indicator bacteria on microbiological
media
MB-based PCR assay for
invA
gene detected 10
genome equivalents in 3 h
96
RNA aptamers
141
PCR using primers to
tuf
gene
58
Shigella
spp.
Based on detection of fecal coliform indi-
cator bacteria on microbiological media
Triplex PCR and
Vibrio
-specific microarray
143
Multiplex PCR
144
Real-time PCR and NASBA down to 1 cfu mL
−1
(IMO
guidelines) toxigenic
V. choleria
in ballast water
76
Vibrio cholerae
The gold standard is considered to be
culturing on selective media, usually
after enrichment
142
Microarray
51
qPCR combined with propidium monoazide (PMA)
145
Continued
Multiple patho-
gens
Search WWH ::
Custom Search