Biomedical Engineering Reference
In-Depth Information
FIGURE 5.11
Mobius CellReady reactor.
Highlights and benefits • Superior mixing capabilities with highly
reduced shear stress • Innovative sparging device with better oxy-
genation and K
L
a • Adapted for cultivation of suspended or adher-
ent cells at very high densities • Compatible with most cell culture
processes • Scalable customizable system • No need for CIP/SIP
(disposable bag technology) • Avoid cross-contamination risks •
Very low working volume.
The Mobius CellReady 3 L bioreactor is equipped with a marine impeller
(top driven), a microsparger or open-pipe sparger, standard sensors, and an
Applikon ez-Control process control unit. Similar cell densities and anti-
body titers can be achieved in the Mobius CellReady, as in stirred 3 L glass
bioreactors. A comparable approach to the Mobius CellReady represents
New Brunswick Scientific's CelliGEN BLU single-use stirred-tank bioreactor
(Figure 5.11).
CellReady Bioreactor
Orbitally Shaken Single-Use Bioreactors
Orbitally shaken bioreactors are very difficult to study because of the free
movement of surfaces in the bioreactors. The surface-aerated CultiFlask 50
disposable bioreactor, a noninstrumented 50 mL centrifuge tube with a ven-
tilated cap, can deliver K
L
a values of between 5 and 30 per hour at CVs of 10
to 20 mL and agitation speeds between 180 and 220 rpm.
