Biology Reference
In-Depth Information
Table 2
(continued)
Spots in gels
A and B
(Fig.
2
)
Peptide
sequenced
ion score
d
Database
accession ID
a
MS & MS/MS
score
c
Scoring
threshold
e
Protein name
Species
MW (Da)
Peptide count
b
12
Serum albumin
precursor (
Bos taurus
)
Bos taurus
gi|30794280 69278.5
41
2,510
2249
83
13
Ovotransferrin
Gallus gallus
gi|83754919 75807.4
28
1,120
957
83
14
Concanavalin A—jack bean
Canavalia
ensiformis
gi|72333
25556.8
13
1,220
1143
83
15
Carbonic anhydrase II
(
Bos taurus
)
Bos taurus
gi|30466252 29095.7
12
448
391
83
16
Chain A, soybean trypsin
inhibitor
Glycine max
gi|3318877
20082.1
15
633
525
83
17
Superoxide
dismutase
(
Bos taurus
)
Bos
taurus
gi|27807109
15672.8
13
952
855
83
18
Carbonic anhydrase II
(
Bos taurus
)
Bos taurus
gi|30466252 29095.7
9
609
572
83
19
Concanavalin A—jack bean
Canavalia
ensiformis
gi|72333
25556.8
6
669
650
83
20
Ubiquitin
(
Scyliorhinus
torazame
)
Scyliorhinus
torazame
gi|10719701
19471
10
584
54
a
The protein sequence was searched at “
http://www.ncbi.nlm.nih.gov/
”
b
Number of observed peptides matching the theoretical digest of the identifi ed protein
c
Combined score of the quality of the peptide mass fi ngerprint match and MS/MS peptide fragment ion matches
d
Score of the quality of MS/MS peptide fragment ion matches only
e
Signifi cant score threshold. A hit with an “MS and MS/MS score” or an “ion score” above this value is considered a signifi cant identifi cation (
p
< 0.05)
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