Analysis of Proteins Using DIGE and MALDI Mass Spectrometry - Difference Gel Electrophoresis (DIGE): Methods and Protocols - page 57

Table 2
Results of protein identifi cation by MALDI MS from labeled and unlabeled protein lysates. Two pairs of proteins ( underline )
were incorrectly identifi ed

Spots in gels
A and B
(Fig. 2 )

Peptide
sequenced
ion score d

Database
accession ID a

MS & MS/MS
score c

Scoring
threshold e Protein name Species MW (Da) Peptide count b

1 Ovotransferrin Gallus gallus gi|83754919 75807.4 39 2,010 1735 83

2 Serum albumin precursor

( Bos taurus )

Bos taurus gi|30794280 69278.5 41 2,170 1887 83

3 Ovotransferrin Gallus gallus gi|83754919 75807.4 26 1,020 916 83

4 Concanavalin A—jack

bean

Canavalia
ensiformis

gi|72333 25556.8 13 1,190 1121 83

5 Carbonic anhydrase II

( Bos taurus )

Bos taurus gi|30466252 29095.7 11 377 326 83

6 Chain A, soybean

trypsin Inhibitor

Glycine max gi|3318877 20082.1 18 1,120 977 83

7 Superoxide dismutase

(Bos taurus)

Bos taurus gi|27807109 15672.8 11 872 791 83

8 Carbonic anhydrase II

( Bos taurus )

Bos taurus gi|30466252 29095.7 10 717 674 83

9 Concanavalin A—jack

bean

Canavalia
ensiformis

gi|72333 25556.8 6 562 522 83

10 Ubiquitin ( Drosophila

melanogaster )

Drosophila
melanogaster

gi|158767 8540.0 7 378 54

11 Ovotransferrin Gallus gallus gi|83754919 75807.4 44 2,300 1929 83

(continued)