Differential Gel-Based Proteomic Approach for Cancer Biomarker Discovery Using Human Plasma - Difference Gel Electrophoresis (DIGE): Methods and Protocols - page 232

Table 2
List of 43 differentially expressed proteins (normal vs. HCC) identifi ed by nanoLC-MS/MS

Ratio
(HCC/Nor) Spot. # GI # Protein name p value MW p I Score Pep. Match Cov. (%)

Decreased
1 gi|179619 Plasma protease (C1) inhibitor

precursor

−3.23 0.01 55,182 6.09 72 5 3

2 gi|179619 Plasma protease (C1) inhibitor

precursor

−2.41 0.02 55,182 6.09 174 6 9

10 gi|112910 Alpha-2-HS-glycoprotein −7.11 0.00005 39,324 5.43 246 111 12

11 gi|112910 Alpha-2-HS-glycoprotein −5.09 0.0017 39,324 5.43 96 3 12

12 gi|619383 Apolipoprotein D 1 apoD −2.19 0.02 27,993 5.14 141 11 12

14 gi|130675 Serum paraoxonase/arylesterase 1 −7.27 0.0027 39,749 5.08 189 17 14

20 gi|5776545 Tax1-binding protein, TXBP151 −2.99 0.012 86,251 5.31 49 4 1

21 gi|186972736 Apolipoprotein C-III −2.25 0.00057 8,765 4.72 176 18 45

22 gi|14277770 Apolipoprotein C-II −2.04 0.007 8,915 4.66 272 58 69

28 gi|130675 Serum paraoxonase/arylesterase 1 −4.82 0.0018 39,749 5.08 87 16 5

30 gi|219978 Prealbumin −2.36 0.026 15,919 5.52 104 5 9

33 gi|219978 Prealbumin −3.00 0.014 15,919 5.52 132 10 9

34 gi|179161 Antithrombin III −2.03 0.0067 52,618 6.32 76 5 8

35 gi|177872 Alpha-2-macroglobulin −2.98 0.002 70,794 5.47 518 71 21

36 gi|388519 Complement factor H-related

protein 1, FHR-1

−4.18 0.044 37,244 7.81 94 4 9

38 gi|182424 Alpha-fi brinogen precursor −2.30 0.0088 69,809 8.26 145 5 9

39 gi|229479 Lipoprotein Gln I −2.02 0.011 28,346 5.27 247 17 28

40 gi|35897 Retinol-binding protein 4 −2.04 0.00079 22,868 5.48 142 16 12

41 gi|62988821 Alstrom syndrome 1, ALMS1 protein −2.67 0.0067 2,78,758 5.03 47 2 0