Chemistry Reference
In-Depth Information
Figure 5.6
(1) Schematic representation for the preparation of the hybrid
dye-doped silica nanoparticle. (2) (a) dependence of per particle
r
values on the number of deposited Gd-DOTA oligomer
1 on NPO. (b)
and
r
1
2
T1-weighted magnetic resonance images of HT-
29 cells that have been incubated with various nanoparticles.
From left to right: control cells without any nanoparticle, cells
with NP3B particles. Cells with NP3B particles that have been
noncovalently functionalized with K
T
1
RGD and cells with NP3B
particles that have been noncovalently functionalized with
K
7
GRD, (c-j) phase contrast optical (c, e, g, and i) and LSCFM
microscopic images (d, f, h, and j) of HT-29 cells that have been
incubated with various nanoparticle: control cells without any
particles (c and d), cells with NP5B particles (e and f), cells with
NP5B particles that have been noncovalently functionalized
with K
7
RGD (g and h), and cells with NP5B particles that have
been noncovalently functionalized with K
7
GRD (i) [62].
7
. also reported multifunctional single-core multiple-
shell Ru(bpy):Gd(III)/SiO
Santra
et al
nanoparticles, which were photostable,
radio-opaque, and paramagnetic [63]. Two silica layers were coated
onto the silica core, sequentially namely, hydrated inner silica shell
and outer silica shell. Highly sensitive and photostable fluorescent dye
Ru(bpy) was first encapsulated into silica core and paramagnetic Gd
ions were then bound to TSPETE in the hydrated inner silica shell via
the microemulsion method. Another silica shell was then coated on
with primary amino groups for bioconjugation. Dye molecules gave
orange-red fluorescence by UV excitation, and magnetic resonance
data showed efficient water exchange with the Gd
2
ions, although
they were embedded inside the amorphous silica shell.
3+
