Biology Reference
In-Depth Information
1.4 10
8
1.2 10
8
1.0 10
8
8.0 10
7
6.0 10
7
4.0 10
7
*
2.0 10
7
0
wBruAus
wBruCon
wBruOri
FIGURE 18.5
Comparison of the population density of wBruCon, wBruOri, and wBruAus between female
and male of newly emerged adults of
C. chinensis
in terms of
wsp
copies per dry weight (mg) of insect (
n
=
10; error bars show standard deviation). Asterisk shows statistically signiÝcant difference (Mann-Whitney
U
-test,
p
< 0.01). [From Ijichi, N., Kondo, N., Matsumoto, R., Shimada, M., Ishikawa, H., and Fukatsu, T.
(2002).
Appl. Environ. Microbiol.
68:
4074Ï4080.]
10
10
10
8
10
6
10
4
10
2
0
egg
FIGURE 18.6
Infection dynamics of wBruCon, wBruOri, and wBruAus throughout the developmental course
of
C. chinensis
in terms of
wsp
copies per individual insect. Diamonds, wBruCon; squares, wBruOri; triangles,
wBruAus (
n
= 10; bars show SD). Pupae and adults were females. Sex was not distinguished at the other
stages. [From Ijichi, N., Kondo, N., Matsumoto, R., Shimada, M., Ishikawa, H., and Fukatsu, T. (2002).
Appl.
Environ. Microbiol.
68:
4074Ï4080. With permission.]
equivalent per insect). In late eggs and Ýrst-instar larvae, the populations of wBruCon, wBruOri,
and wBruAus were at almost the same level, around 6 10
5
wsp
copies equivalent per insect. At
second larval instar and later stages, the populations of wBruCon and wBruOri were at comparable
levels, whereas the populations of wBruAus were consistently and signiÝcantly smaller. In prepupae
and pupae, the populations of the
Wolbachia
strains reached a plateau: wBruCon, wBruOri, and
wBruAus were around 3 10
8
, 2 10
8
, and 5 10
7
wsp
copies equivalent per insect, respectively.
WOLBACHIA
DENSITY IN TISSUES OF
C. CHINENSIS
ADULTS
To quantitatively investigate the localization of wBruCon, wBruOri, and wBruAus in
C. chinensis
tissues, dissected tissues and organs from adult insects were subjected to quantitative PCR analysis.
To make a comparison between different tissues and organs, the
wsp
gene copy number of
Wolbachia
