Biomedical Engineering Reference
In-Depth Information
exsanguination. The liver is a well-perfused organ that is clearly
visible during the PBS perfusion. Liver clearance, the paling
color change that results from blood leaving the liver, will
indicate the quality of the exsanguination. Once liver clearance
is achieved, stop the pump.
Mouse
Weight (g)
Blood volume (mL)
20
1.6
25
2.0
30
2.4
11. Use the bone cutting shears to remove the rodent head at the
neck.
12. To collect retina and optic nerve tissue, curved toothed forceps
are used to enucleate the eye. With either the left or right eye
pointed up, press down the flesh above and below the eye
socket to proptose the eye.
13. Place the curved toothed forceps behind the globe and close
them. Pull straight up, bringing the eye and optic nerve out of
the socket.
Rat
Weight (g)
Blood volume (mL)
250
17.5
300
21.0
400
28.0
14. While still holding the eye clamped in the enucleating forceps,
use spring scissors to enter the cornea anterior to the ora
serrata. Cut along the circumference of the cornea; discard
the cornea.
15. The iris is often removed as a result of corneal removal. Use
forceps to pull out the lens.
16. Finally, with the globe pressed against a flat surface (gloved
fingertip or sterile surface) place forceps at the back of the
globe, press down and forward. This action will ease the retina
out of the eyecup so that it can be collected. Place the retina in
PBS for further dissection or until ready for the pre-analysis
processing.
17. Once the retina is removed, use scissors to cut the optic nerve
off the posterior eyecup. Place it in PBS for further dissection
or until ready for the pre-analysis processing.
