Biomedical Engineering Reference
In-Depth Information
Fig. 1 To analyze drug concentrations in the eye, the key is to accurately dissect
the correct tissue types. For the retina, the eye is removed after which the retina
is dissected out. Similarly, the optic tracts and the superior colliculus can be
dissected to analyze the distribution of drug in these areas
5. Use hemostats in one hand grasp the xiphoid process and
elevate the anterior rib cage in order to cut across the dia-
phragm with the scissors using the other hand. This protects
the heart from possible damage by the scissors.
6. Make cuts perpendicular to the ribs that extend up toward the
neck, creating a thorax flap that can be laid open to expose
the heart. Lay the hemostats in such a way as to keep the thorax
flap open.
7. Use scissors to cut into the right anterior chamber of the heart.
8. Use forceps to hold the heart while placing the pump needle
into the apex of the left ventricle.
9. Turn on the pump. Monitor the pump tubing so that no air
bubbles are delivered to the heart.
10. Mouse blood volume is 77-80
L/g; rat blood volume is
55-70 mL/kg. Run the pump so the volume of PBS through
the rodent is at least two times the blood volume, but bear in
mind that liver clearance is always a better indicator of quality of
μ
