Biology Reference
In-Depth Information
FIGURE 5.4
(A) Downregulation of ZEB1 and relative changes in
-tubulin mRNA in MDA-MB-231
cells. SiRNA technology was used to reduce ZEB1 mRNA levels. Quantitative qRT-PCR
was carried out to measure changes in mRNA levels for ZEB1 and b-tubulin isotypes.
The bars represent the fold difference in mRNA: ZEB1 SiRNA-treated cells/scrambled
sequence-treated cells. The data are representative of three independent experiments.
(B) Upregulation of ZEB1 and relative changes in
b
b
-tubulinmRNA. A TrueClone
(OrigGene,
™
Rockville, MD) plasmid expressing human ZEB1 variant 1 (Accession number
NM_001128128.1) or empty vector were used to upregulateZEB1 incell cultures. Quantitative
qRT-PCR was used to measure changes in mRNA for ZEB1 and
-tubulin isotypes. The bars
represent the fold difference in mRNA: ZEB1 transfected cell copy number/empty vector
transfected cell copy number. The data are representative of three independent experiments.
b
