Biology Reference
In-Depth Information
FIGURE 5.2
Schema showing the effects of miR-100 on the Akt/mTOR pathway and
-tubulin isotypes.
MiR-100 was shown to reduce levels of phosphorylated Akt and mTOR, signals implicated in
tumor progression (
Nagaraja et al., 2010
). In MCF7 breast cancer cells, miR-100 regulates
b
b
-tubulin isotype classes I, IIA, and V (
Lobert et al., 2011
). Paclitaxel treatment reduces
miR-100, and this result has implications for drug resistance and the potential for
b
-tubulin
isotypes to be used as biomarkers for tumor progression.
FIGURE 5.3
Comparative qRT-PCR for miR200c. MDA-MB-231 cells were treated with 40 nM paclitaxel
for 24 h. Data were normalized to SCA17 as the housekeeping gene.
