Investigating Tubulin Posttranslational Modifications with Specific Antibodies - Methods in Cell Biology - page 250

Table 16.1

Overview of antibodies detecting tubulin PTMs described in this work

Commercial
availability Name Citations Type Modification Comment

6-11B-1 LeDizet and Piperno (1991) ,

Piperno and Fuller (1985)

Monoclonal
mouse IgG2b

Acetylation K40
on a-tubulin

Raised against flagellar tubulin
from sea urchin

#T6793,
#T7451
(Sigma)

YL1/2 Cumming, Burgoyne, and

Lytton (1984) , Kilmartin,
Wright, and Milstein (1982)

Monoclonal
rat IgG2a

C-terminal -EEF
(slightly preferred)
and -EEY on
a-tubulin

Initially raised against purified
yeast tubulin (-EEF)

#MAB1864
(Millipore)

Anti-
detyr-
tubulin

Paturle-Lafanechere et al.
(1994)

Polyclonal
rabbit

C-terminal -GEE
on a-tubulin

Raised against -CGEEEGEE #AB3201

(Millipore)

Anti-
△

Paturle-Lafanechere et al.
(1994)

Polyclonal
rabbit

C-terminal -GE
on a-tubulin

Raised against -CEGEEEGE #AB3203

(Millipore) 2-

tubulin

GT335 Wolff et al. (1992) Monoclonal

mouse IgG1k

g-Linked E n side
chain (n¼

Detects most glutamylated
a- and b-tubulin

#AG-20B-
0020
(Adipogen)

1, 2,

3 ) on modified E

...

polyE Rogowski et al. (2010) ,

Shang, Li, and Gorovsky
(2002)

Polyclonal
rabbit

C-terminal -E n
(n

Detects polyglutamylation Not available

3)

1D5 R ¨ diger et al. (1999) , Warn,

Harrison, Planques, Robert-
Nicoud, and Wehland
(1990)

Monoclonal
mouse IgG1

C-terminal -E n
(n2)

Detects polyglutamylation and
detyrosination on most a-tubulin
isoforms (C-terminal sequence
-EE)

#302 011
(Synaptic
Systems)

Br ´ et al. (1996) , Callen et al.
(1994) , Levilliers, Fleury, and
Hill (1995)

TAP952 Monoclonal

mouse IgG1

g-Linked G side
chain (n¼1) on
modified E

Detects strictly monoglycylation Currently not

available

AXO49 Br ´ et al. (1996) , Callen et al.

(1994) , Levilliers et al. (1995)

Monoclonal
mouse IgG1

g-Linked G n side
chain (n

Detects polyglycylation Currently not

available 3) on

modified E

polyG Rogowski et al. (2009) ,

Shang et al. (2002)

Polyclonal
rabbit

C-terminal polyG
chains

Detects polyglycylation Currently not

available

Adapted from Janke and Bulinski (2011)