Biomedical Engineering Reference
In-Depth Information
(c)
175
pH
(Induction)
125
75
175
DO%
125
75
175
Feed-2
125
75
-
+
Figure
5.11.
(continued).
TABL E 5.4. Worst and Best Case Operation Conditions and Performance Characteristics
During the Production Stage in the Case Study
Worst Case
Set Point
Best Case
Growth
Phase
Production
Phase
Growth
Phase
Production
Phase
Growth
Phase
Production
Phase
Operational
Parameter
DO%
þ
þ
0
0
Temperature
þ
þ
0
0
Feed-1
N/A
0
N/A
N/A
pH
þ
þ
0
0
þ
Induction duration
N/A
N/A
0
N/A
Performance Parameter
Worst Case
Set Point
Best Case
Product yield
57
5
100
4
114
4
Product quality 1
198
9
100
18
76
2
Product quality 1
(post-AEX)
226
9
110
14
n.d.
Product quality 2
93
13
100
2
87
7
Product quality 2
(post-AEX)
550
17
417
93
n.d.
Data have been normalized against the average bench scale performance at set point operating conditions at the
end of the production phase. N/A, not applicable; n.d., not determined; AEX, anion exchange chromatography.
The symbols
, 0, and
þ
indicate the low end, the set point, and the high end of the normal operating range,
respectively.
