Biomedical Engineering Reference
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Fig. 9.5 Bioorthogonal nanoparticle detection (BOND) .( a ) The method is based on the Diels-
Alder cycloaddition between trans-cyclooctene (TCO) and tetrazine (Tz). ( b ) Cells are prelabeled
with TCO antibodies and targeted with Tz-MNPs. The antibody provides sites for mutliple
MNP couplings. ( c , d ) Compared to the direct targeting with MNP-antibody conjugates, BOND
method enabled higher MNP loading on target cells as confirmed by fluorescent ( c )andNMR
( d ) measurements (Reproduced from [ 27 ]. Copyright 2010 Nature Publishing Group)
Second, miniaturized NMR probes (coils) produce much stronger radio-frequency
(RF) magnetic fields per unit current, leading to higher signal-to-noise per unit
sample volume [ 53 ]. Third, with smaller RF coils, the requirement for spatial
homogeneity of static magnetic fields becomes less stringent, making it possible
to use small, portable magnets [ 22 ]. The entire measurement system can be realized
as a portable device for point-of-care operations.
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