Biomedical Engineering Reference
In-Depth Information
Fig. 9.5
Bioorthogonal nanoparticle detection (BOND)
.(
a
) The method is based on the Diels-
Alder cycloaddition between trans-cyclooctene (TCO) and tetrazine (Tz). (
b
) Cells are prelabeled
with TCO antibodies and targeted with Tz-MNPs. The antibody provides sites for mutliple
MNP couplings. (
c
,
d
) Compared to the direct targeting with MNP-antibody conjugates, BOND
method enabled higher MNP loading on target cells as confirmed by fluorescent (
c
)andNMR
(
d
) measurements (Reproduced from [
27
]. Copyright 2010 Nature Publishing Group)
Second, miniaturized NMR probes (coils) produce much stronger radio-frequency
(RF) magnetic fields per unit current, leading to higher signal-to-noise per unit
sample volume [
53
]. Third, with smaller RF coils, the requirement for spatial
homogeneity of static magnetic fields becomes less stringent, making it possible
to use small, portable magnets [
22
]. The entire measurement system can be realized
as a portable device for point-of-care operations.
