Biology Reference
In-Depth Information
FIGURE 10.1
Processing and membrane association of HCV and DENV proteins. (A) HCV genome is a
positive-sense single-strand uncapped RNA molecule of 9.6 kb. Genome translation
yields a polyprotein precursor which is co- and post-translationally processed into 10
proteins. (B) DENV genome is a positive-strand capped RNA molecule of
11 kb. Genome
translation and subsequent processing of the polyprotein result in 11 proteins.
associated with membranes (
Fig. 10.1
). In this chapter, we will focus only on viruses
and provide a list and review of the methods used to study the interactions between
these viruses and host LDs.
Interactions between viruses and LDs
The implication of LDs in the lifecycle of viruses was first documented in 2007
with hepatitis C virus (HCV) (
Miyanari et al., 2007
), which continues to be the best
understood example of how viruses interact with LDs. Thus, this chapter will focus
predominantly on the methods used to study the interplay between HCV and LDs.
Other viruses, mainly RNA viruses, also rely on LDs for part of their replication. These
include dengue virus (DENV), rotavirus (RV), orthoreovirus, and hepatitis B virus
(HBV). We will also provide examples of the different types of interactions described
thus far between viruses and cellular LDs, which are summarized in
Table 10.1
.
