Biology Reference
In-Depth Information
SO
3
−
SO
3
−
NH
2
+
N
+
H
2
N
O
N
O
O
NH
R=
COO
−
COO
−
O
−
O
3
S
SO
3
−
HN
HN
R
O
R
O
DIBO
Alexa 488
Alexa 594
−
O
3
S
SO
3
−
−
O
3
S
SO
3
−
N
+
N
+
N
N
−
O
3
S
SO
3
−
−
O
3
S
SO
3
−
O
O
NH
NH
R
R
Alexa 555
Alexa 647
SCHEME 15.3
Fluorescent labels for strain-promoted azide-alkyne cycloaddition (SpAAC). The structure of
Alexa 555 is inferred from Alexa 647 (
Cordes, Maiser, Steinhauer, Schermelleh, & Tinnefeld,
2011
). Alexa 488 and 594 are rhodamines, whereas Alexa 555 and 647 are cyanines.
FF01-525/30-25 (BP5)
FF01-605/15-25 (BP3)
FF01-676/29-25 (BP6)
CHROMA:
ZET405/488/635NF EM (NF1)
ZET561NF EM (NF2)
EDMUND OPTICS:
750 nm dichroic shortpass filter (25
36, #69-219)
APPLIED SCIENTIFIC INSTRUMENTATION:
CRISP Autofocus (with 780 nm LED)
PZ-2000-XY-Piezo Z system (closed loop XY stage with rotary encoders and
150
m Piezo Z top plate)
m
PHOTOMETRICS:
Evolve 512 eXcelon EMCCD
QuadView Imager
