Biology Reference
In-Depth Information
FIGURE 10.3
Sample FCS recordings for YFP-tagged beta2-adrenergic and dimeric CD-28 receptors
diffusing within the plasma membrane of HEK293 cells. (A) fluorescence intensity trace; (B)
autocorrelation analysis of the fluorescence intensity trace; (C) photon-counting histogram;
(D) residuals of the curve fit.
a very fast component characteristic of the photophysical properties of YFP
(50-100
m
s) and a slower component representing the translational diffusion of the
receptor within the plasma membrane. Once the diffusion time (t
D
) for the receptor
has been determined, then the diffusion coefficient (
D
) can be calculated using
