Biology Reference
In-Depth Information
Table 9.3
Treatment methods based on the use of
Bacillus
sp. for fi sh waste remediation (parameters, quality control and results)
No.
Kind of
waste
Treatment
Parameters
Methodology
Quality control
methods
Results
References
1.
Prawn
nursery
wastewater
Microbial
remediation
using
Bacillus
subtilis
pH, water
temperature,
disswolved
oxygen, wa-
ter salinity,
TAN, nitrite,
nitrate,
phosphate,
COD
Filtation by sand
fi lter, sterilization
by ultraviolet
reactor, addition
of
Bacillus subtilis
under conditions
of continuous
aeration, fi ltation
through sand
fi lter
1. pH, water temperature, dissolved
oxygen: by handheld meters model pH
315i or Oxi 315i
2. Water salinity: using optical refracto-
meter
3. TAN, nitrite, nitrate, phosphate: spec-
trophotometrically
4. COD: by oxidization of organic matter
with potassium permaganate ac-
cording to standard methods (Wu et
al.,1980)
5. COD removal up to 57.5 ±
5.5%, and DOM degradation
after 48th
6. TAN removal decrease from
day 2 to day 5
7. Glucose and/or phosphate
addition resulted in TAN
removal (>85%)
8. Vitamin mixture and/or
micro-elements solution
addition did not affect TAN
removal
9. Optimal rates of C/N or N/P
for wastewater remediation
5.4/1 or 5-7/1, respectively
Liu and
Han, 2004
2.
Basal shrimp
feed
Bioreme-
diation with
Bacillus sp.
YB-030518
and
Bacillus
sp.
YB-034325
Probiotic
cell surface
hydropho-
bicity
Two strains of
Bacillus sp
. (1%
inoculation) were
added to basal
shrimp feed at
30°C for 96h
Probiotic cell surface hydrophobicity: by
bacterial adherence to hydrocarbons as-
say, which is based on the partitioning of
cells in a two-phase system (Rosenberg et
al., 1980; Zhang and Miller, 1994)
1. No signifi cant differences
in cell wall hydrophobicity
between the two strains of
Bacillus sp
. (pH 5.5,7.0 and
8.5, at 5°C and 20°C)
2. Bacillus YB-034325 had
higher bioremediation capa-
bility (%) at 48h and 96h
Wang and
Han, 2007
3.
Recirculat-
ing-water
Aquaculture
water reme-
Addition of
Bacillus subtilis
and
1. Total ammonia nitrogen
concentrations 5.4mg/L
2.COD < 40.8mg/L
Chen and
Chen, 2001
