Biology Reference
In-Depth Information
4.
Fish bone
wastes
Treatment
with enzymes
L and K
originated
from
Bacillus
species
Organic
matter, fat
content, pro-
tein content
molecular
weight,
water reten-
tion capacity,
water va-
porozation
potential,
extract
odor, skin
patch test,
peptidee
molecular
weight,
amino acid
composition,
physiologi-
cal activities
Pre-treated (fat
removal, deshing),
treated enzy-
matically, agitated
(200rpm, 60min,
60°C, Ph8.0),
centrifuged
(800xg,10min)
1. Organic matter the amount of a sample:
vaporized during incineration at
600°Cfor 30 min
2. Fat content: by Soxhlet extrector
3. Protein content: by Lowry-Folin
method (Lowry et al., 1951)
4. Molecular weight: by Laemmli method
(Laemmli,,1970)
5. Water retention capacity: by direct
application of human skin and using
hygrometer
6. Water vaporozation potential: by direct
application of human skin and using
Tewameter
7. Extract odor: sample dilution with pH
adjustments
8. Skin patch test: by the Association
of Industrial Information's method
(Hayakawa et al., 1975)
9. Peptide molicular weight: mean degree
of polymerization and amino acid
composition
10. Amino acid composition: hydrolysis
of a sample under both acidic and
alkiline conditions
11. Physiological activities: antioxidant
activity (by luminol-enhanced chemi-
luminescence). ACE inhibitory activity
(by a modifi ed method of Cushman
and Cheung,1971)
1. Protein recovery, <53%
2. High collagen degradation
effi ciency by enzyme L
(85.5%) compared to enzyme
K (69.2%)
Morimura
et al., 2002
