Biomedical Engineering Reference
In-Depth Information
22
Detection of Hanta, Influenza A, and Parainfluenza
Virus
Ebtisam S. Wilkins and Ravil A. Sitdikov
CONTENTS
22.1 Introduction ....................................................................................................................598
22.1.1 Hanta Virus ........................................................................................................598
22.1.2 Avian Influenza ................................................................................................598
22.2 Sensor Design and Immunoassay System ..................................................................600
22.2.1 Detection of Hanta Virus ................................................................................600
22.2.2 Detection of Parainfluenza and Influenza A Viruses ..................................601
22.2.3 Immunoassay Scheme and Amperometric Detection ................................601
22.2.4 Optimization of the Assay Parameters ..........................................................601
22.2.5 Optimization of the Amperometric Measurement Stage ............................601
22.2.5.1 The Cyclic Voltammetry of Horse-Radish Peroxidase
and Alkaline Phosphatase Enzymatic Products ........................601
22.2.5.2 Selection of a Working Potentials ................................................602
22.3 Experimental Design ......................................................................................................604
22.3.1 Reagents and Materials ....................................................................................604
22.3.1.1 For Hanta Virus ..............................................................................604
22.3.1.1.1 Experimental Procedure ............................................604
22.3.2.1 For Influenza A Virus and Parainfluenza Virus ........................605
22.4 Results and Discussion for Hanta Virus Test ..............................................................605
22.4.1 Field Test of Hemolysis Blood Samples ........................................................606
22.4.2 Effect of H
2
O
2
Used in the Substrate ..............................................................608
22.4.3 Alkaline Phosphatase vs. Horse-Radish Peroxidase ..................................608
22.4.4 Nafion Layer ......................................................................................................609
22.4.5 Result and Discussion of Influenza Samples ................................................610
22.5 Conclusions ......................................................................................................................614
References ....................................................................................................................................614
597
