Gene Therapy Approaches Using Reproducible and Fully Penetrant Lentivirus-Mediated Endogenous Glioma Models - Gene Delivery and Therapy for Neurological Disorders - page 346

(d) Xylazine (Animal Health International, http://www.pvpl.
com )
(e) Atipamezole (Orion Corp., http://www.orion.fi )
(f) Buprenorphine (Animal Health International, http://
www.pvpl.com )
(g) Ganciclovir (GCV, Roche Pharmaceuticals, http://www.
rocheusa.com )
5. Betadine (Bruce Medical, cat# FR-2100-88)
6. Virkon S (DuPont, www.dupont.com)
7. Puralube ophthalmic ointment (Fera Pharmaceuticals, http://
www.ferapharma.com )
8. Warm lactated Ringer's solution (Hospira Inc., cat#
0409-7953-30)
9. Stereotactic frame with rat adapters and blunt ear bars
(Stoelting Inc.)
10. Stereomicroscope (e.g., Zeiss Stemi 1000 zoom) equipped
with 16× eyepieces and 0.4× auxiliary objective lens and
mounted on hinged coupling arm on a heavy foot stand (or
equivalent)
11. Electric drill with 1.75-mm and 0.6-mm drill bit (Stoelting
Inc., cat# 58610).
12. Digital scale for animal weighing (Harvard Apparatus, cat#
724586)
13. Fiber-optic illuminator with twin gooseneck pipes (Leica Inc.)
14. 10 l, 26-G Hamilton syringe with needle (Fisher, cat# 701RN)

15. 5-10

ʼ

ʼ l, 33-G Hamilton syringe with needle (Fisher, cat#

75RN)
16. 3-0 nylon sutures (Ethicon Inc., cat# 1663H)
17. Petri dish, plastic (BD Pharmingen, 353002)
18. Surgical equipment:
(a) Surgical shavers (Stoelting Inc., cat# 58610)
(b) Scalpel and blades (Cardinal Health, cat# D2862-15)
(c) Skin retractors (Fine Science Tools Inc., mouse cat#
17000-03, rat cat# 17000-04)
(d) Cotton swabs (VWR, cat# 89031-270)
(e) Curved and straight forceps
(f) Holding scissors
(g) Sharp scissors
(h) Sterile gauze (VWR, cat# 95038-720)
(i) Bead sterilizer (VWR, cat# IS-350)
(j) Red lamp (Sylvania, cat# 14663)