Biomedical Engineering Reference
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Fig. 1
Representative histology from tumors induced using lentiviral vectors expressing oncogenes PDGF and
HRAS-V12. (
a
) Nissl staining of large glioblastoma (indicated by âTâ). Areas of tumor hemorrhage indicated
(
arrows
). (
b
) Ki67 staining (
arrow
) showing moderate to high proliferation index. (
c
) Nissl staining showing
pseudopalisades (
arrows
) surrounding necrosis (
asterisk
), a hallmark of human GBM. (
d
) Immunostaining of
neuronal tissue (NeuN,
green
) being infi ltrated by tumor with vasculature immunoreactive with Von Willebrand
factor (VWF,
red
).
Yellow dotted line
delineates tumor border as evident by increased cellular density with DAPI
staining. This infi ltrative high-grade tumor entraps healthy neurons (
arrow
) (color fi gure online)
2
Materials
1. Lentiviral vectors of interest, i.e., pLVX-Puro-hPDGFb,
pLVX-Puro-Flag-HRAS-V12, and pLVX-Puro-HA-AKT.
2. Therapeutic and control adenoviral vector, i.e., Ad-TK and
Ad-0.
3. Adult rat (250 g body weight, Fisher or Sprague-Dawley
Strain, Charles River Inc.,
http://www.criver.com
).
4. Drugs:
(a) Ketamine (Bioniche Teoranta,
http://www.bionichephar-
(b) Dexmedetomidine (Orion Corp.,
http://www.orion.fi
)
(c) Carprofen (Hospira Inc.,
http://www.hospira.com
)
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