Gene Therapy in Spinal Muscular Atrophy (SMA) Models Using Intracerebroventricular Injection into Neonatal Mice - Gene Delivery and Therapy for Neurological Disorders - page 314

Fig. 8 The sequential steps in ICV injection of the mice brain are illustrated. ( a ) The proper holding of the needle
and the syringe during injection. ( b ) Grasping the pup's head for the visualization of the anatomical structure
of the mouse brain using a fi ber-optic light. ( c ) Penetration of the needle into the left ventricle. ( d ) Penetration
of the needle into the right ventricle
Fig. 9 A successful ICV injection is verifi ed by monitoring the dissemination of the dye in the brain and spinal
cord. ( a ) Dissemination of the dye into the right ventricle within minutes after injection into the left ventricle.
( b ) Dissemination of the dye into the spinal cord within a few hours postinjection