Biomedical Engineering Reference
In-Depth Information
Fig. 7
The steps to upload the injection solution into the needle are illustrated. (
a
) Needle is attached to the
syringe via long tubing. (
b
) Uptake of the injection solution into the needle by placing the needle inside the
injection solution and pulling the plunger of the syringe. (
c
) Uploaded needle containing approximately 5-7
ΚΌ
l
of the injection solution. (
d
) Uploaded needle is attached to the syringe with pulled back plunger
contact/faqs/pipette_cookbook.pdf
.
The needles are categorized
by numbers representing the specifi c program settings on the
micropipette puller based on the heating temperature, pull
strength, velocity, and time. We have found that the best needles
for the injection of short oligonucleotides are #19. These glass
capillary tubes come very close to 26
3/8
G intradermal bevel needle
(125 mm in length, 1.2 mm outside diameter, and 0.45 mm inside
diameter). For less soluble materials, such as viral preps, #11 nee-
dles are more effi cient. In general, greater pulling force and speed
result in fi ner and sharper tips. The settings for these particular
needles are as follows:
#19
Heat = 295
Pull = 40
Velocity = 20
Time = 20
#11
Heat = 320
Pull = 150
Velocity = 100
Time = 150
The parameters are adjusted based on the specifi c type of the
materials to be injected and may require testing different needle
numbers to identify the most fi tting needle type. Number 19
needles are shown in Fig.
6a
.
Search WWH ::
Custom Search