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Senders (cyan filter)
Receivers (yellow filter)
Overlay of images
Figure 7.23
Microscope fluorescence images of overlapping communicating cells
(4
×
objective).
The second visual observation experiment captured a time-lapse series of im-
ages that illustrate the dynamic behavior of the communications (Figure 7.25).
Sender and receiver cells were grown separately overnight at 37°C, shaking
at 250 rpm, in 2 ml LB Amp/Kan cultures inside 14 ml Falcon polystyrene
tubes, pelleted, and resuspended in 100
µ
l fresh LB Amp/Kan. We spotted two
7
.
0
l droplets of receiver cells on an LB Amp/Kan and 1 mM IPTG agar plate,
anda7
.
0-
µ
l droplet of sender cells was spotted near the two receiver droplet.
For this experiment, the plate was incubated at room temperature under the
µ
