Biomedical Engineering Reference
In-Depth Information
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Figure 2.13
Immunostaining and fluorescence ratio images (FRI) of focal adhesion
proteins. REF52 cells transfected with GFP-integrin b3 were fixed and
immunostained with primary antibody against vinculin, followed by
Cy5-conjugated secondary antibodies. Actin filaments were visualized
with phalloidin-TRITC. Cells on 58 nm and 108 nm RGD nanopatterns
were observed at 3 h and 24 h after plating. The rows present the images
with integrin b3 in red, vinculin in blue, and actin in green. The last row
shows the ratio between integrin b3 and vinculin intensities. FRI are
presented in a spectrum scale as indicated in the lookup table.
(Reprinted by kind permission of Cell Press, Biophysical Society.)
spatiality,
46
the lack of robustness of such films has been cited as constituting a
serious problem for this approach.
47
An attractive alternative is provided by
hydrosilylation chemistry, which results in the attachment of an organized
monolayer with strong Si-C covalent bonds.
48
An example of RGD
attachment to such a surface is the production of a mixed monolayer on silicon
of oligo (ethylene oxide) species.
49
In this experiment the main focus was the
