FastPCR Software for PCR, In Silico PCR, and Oligonucleotide Assembly and Analysis - DNA Cloning and Assembly Methods - page 283

Table 3
(continued)

Primer ID Sequence (5 ′ -3 ′ ) Length (nt) T m (°C) dG, kcal/mol T m 3 ′ end (°C) CG % LC PQ

Forward primer ID Sequence (5′-3′) T m (°C) PQ Reverse primer ID Sequence (5′-3′) T m (°C) PQ PCR Fragment

Size (bp)

T opt (°C)

1F1_1_8-27 gcggatcacttgaggtcagg 58.9 87 1R3_1_301- 320 ctctggccatcccagttcaa 57.6 80 313 63

1F1_1_8-27 gcggatcacttgaggtcagg 58.9 87 1R4_1_291- 310 cccagttcaagccatcccct 60.2 76 303 65

1F2_1_49-68 caacgtggagctaggtatgg 56.0 80 1R1_1_409- 429 gaagagcctgagtgggcacaa 60.0 85 381 62

1F2_1_49-68 caacgtggagctaggtatgg 56.0 80 1R3_1_301- 320 ctctggccatcccagttcaa 57.6 80 272 62

1F2_1_49-68 caacgtggagctaggtatgg 56.0 80 1R4_1_291- 310 cccagttcaagccatcccct 60.2 76 262 62

1F3_1_109-130 caggagaatcacttcaacctgg 56.4 87 1R1_1_409- 429 gaagagcctgagtgggcacaa 60.0 85 321 62

1F3_1_109-130 caggagaatcacttcaacctgg 56.4 87 1R3_1_301- 320 ctctggccatcccagttcaa 57.6 80 212 62

1F3_1_109-130 caggagaatcacttcaacctgg 56.4 87 1R4_1_291- 310 cccagttcaagccatcccct 60.2 76 202 62

1F4_1_134-154 gcggaggttgcagtgaaccga 62.6 90 1R1_1_409- 429 gaagagcctgagtgggcacaa 60.0 85 296 66

1F4_1_134-154 gcggaggttgcagtgaaccga 62.6 90 1R2_1_311- 333 tgtccttgctcagctctggccat 62.3 83 200 68

1F4_1_134-154 gcggaggttgcagtgaaccga 62.6 90 1R3_1_301- 320 ctctggccatcccagttcaa 57.6 80 187 63

1F4_1_134-154 gcggaggttgcagtgaaccga 62.6 90 1R4_1_291- 310 cccagttcaagccatcccct 60.2 76 177 65

1F5_1_153-172 gagaccgcgccactgcactc 64.3 73 1R2_1_311- 333 tgtccttgctcagctctggccat 62.3 83 181 68

1F5_1_153-172 gagaccgcgccactgcactc 64.3 73 1R4_1_291- 310 cccagttcaagccatcccct 60.2 76 158 65