Biomedical Engineering Reference
In-Depth Information
Table 3
Program output of the primer design
Primer ID
Sequence (5
′
-3
′
)
Length (nt)
T
m
(°C)
dG, kcal/mol
T
m
3
′
end (°C)
CG %
LC
PQ
1F1_1_6-27
gggcggatcacttgaggtcagg
22
63.0
−29.7
40.0
63.6
88
87
1F2_1_107-132
ggcaggagaatcacttcaacctggga
26
62.5
−33.6
40.2
53.8
89
89
1F3_1_133-154
ggcggaggttgcagtgaaccga
22
64.4
−31.3
43.9
63.6
90
90
1F4_1_153-175
gagaccgcgccactgcactccag
23
67.2
−33.7
42.8
69.6
76
76
1F5_1_166-187
tgcactccagcctgggcgacag
22
66.3
−32.1
49.5
68.2
85
85
1F6_1_176-197
cctgggcgacagcctgagactc
22
64.8
−30.8
41.4
68.2
80
80
1F1_2_859-880
cttacggaggccgagatgggca
22
63.4
−30.6
46.9
63.6
88
87
1F2_2_913-934
tggccaacatggtgaaaccctg
22
60.9
−28.9
41.6
54.5
82
80
1F3_2_956-977
aattagctgggcatggtggcac
22
60.9
−29.1
47.8
54.5
80
73
1F4_2_1013- 1036
tggagctgaaccactgcactccag
24
63.2
−32.3
42.8
58.3
79
79
1R1_1_412-434
acccagaagagcctgagtgggca
23
63.9
−31.7
46.7
60.9
83
83
1R2_1_309-330
ccttgctcagctctggccatcc
22
63.6
−30.0
44.8
63.6
80
80
1R3_1_299-320
ctctggccatcccagttcaagc
22
61.5
−28.9
42.2
59.1
88
80
1R1_2_1206- 1231
agatggggtttcaccatgttggccca
26
64.1
−34.8
44.6
53.8
80
80
1R2_2_1166- 1187
acctcaggtgatccacctgcct
22
61.7
−29.5
44.2
59.1
82
80
1R3_2_1150- 1174
cacctgcctcagcttcccaaagtgc
25
65.3
−34.2
40.6
60.0
84
84
1R4_2_1132- 1157
caaagtgcttggattacaggcgtgag
26
61.0
−33.0
42.4
50.0
93
93
The separated output of a set of primer pair sequences with their theoretical PCR products
(continued)
