Biomedical Engineering Reference
In-Depth Information
7
4
1
3
13
9
5
8
6
2
10
11
12
FIGURE 12.10
Schematic of the experimental setup of the application of MSFBs to the
removal of yeast cells: 1—column; 2—supporting grid; 3—magnetic particles;
4—power supply; 5—feed tank; 6—mixer; 7—euent stream; 8—peristaltic
pump; 9—euent receiver; 10—distilled water; 11—centrifugal pump; 12—
valve; 13—magnetic system. (From Al-Qodah and Al-Shannag 2006.)
of the applications previously described, however, due to their importance,
they are referred in this separate section.
Immunoglobulin G separation
( Ozkara et al. 2004; Karatas et al. 2007;
Ozturk et al. 2007), and
antibody removal
(Odabas et al. 2005) are recent
applications of MSFBs. They represent an important step concerning clini-
cal analysis and the treatment of diseases like lupus and cancer. They are
based in the same process described for protein purification and, while in
immunoglobulin G, functionalized mag-poly (EGDMA-MAH) beads are used,
in the antibody removal functionalized mPHEMA beads are the usual choice.
Separation of cells and nucleic acids
are also important applications of
MSFBs, and, as before, the process is very similar to the one previously
described for protein purification (Al-Qodah and Al-Shannag 2006, 2007).
A schematic drawing of such a process setup is depicted in Figure 12.10, for
the case of yeast cells, where magnetic beads are composed by a magnetite
(Fe
3
O
4
) core, and covered by a stable layer of activated carbon whose function
is to adsorb the cells from the suspension.
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