Biomedical Engineering Reference
In-Depth Information
UV detector
Computer
Magnetic field
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Column
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0
Buffer
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FIGURE 12.9
Experimental setup of a MSFB for protein adsorption. (From Tong and Sun
2003.)
chromatographic technique using continuous supermacroporous monolith has
been used for the direct purification of products.
This chromatographic technique is useful for the selective separation of
proteins and enzymes. There are two typical operation setups: one based on
the use of nanomagnetic particles covered by a functionalized porous polymeric
layer that is assembled in a magnetic bed structure (Cocker et al. 1997), and
the other being composed by a supermacroporous cryogel column containing
embedded ferromagnetic nanoparticles that are acted by the external magnetic
field, a setup that allows to attain flows similar to those obtained in conven-
tional chromatographic columns, having, at the same time, several pores that
selectively delay the target substances (Yao et al. 2006).
Typical application examples of MSFB chromatography in biotechnology
are the chromatographic separation of bovine serum albumin (Yao et al. 2006)
and the anity separation of trypsin from chymotrypsin (Cocker et al. 1997).
12.5.2.4
Novel Separations
In this section, some details will be given on recent applications of MSFBs in
our areas of concern. Some of them could have been considered as being part
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