Biomedical Engineering Reference
In-Depth Information
Figure 13.7
Collagen and elastin microstructure of the porcine carotid artery. Two-photon image 24 mm below
the surface of the porcine carotid intima. Elastin autofluorescence is red; collagen SHG is green. A wavy sheet of
elastin with circular holes throughout envelops the luminal surface of the artery. Individual, bunched-up collagen
fibrils are radially arranged within the inner folds of the elastin lamellae. Scale bar = 20 μm.
Figure 13.8
LDL binds to collagen- and proteoglycan-rich, elastin-poor aortic branch points. (a) Three-
dimensional surface reconstructions of atherosclerosis-susceptible intervertebral branch points along the mouse
thoracic aorta revealing with a ring of exposed collagen immediately surrounding the ostia. Elastin autofluores-
cence is red; collagen SHG is green. Scale bar = 50 μm. (b) LDL binding along the mouse aorta detected by epifluo-
rescence. Alexa 647-tagged LDL bound extensively to a circular region around intervertebral branch points. Scale
bar = 500 μm. Zoom scale bar = 100 μm. (c) Colocalization analysis of LDL binding at porcine coronary arte-
rial branch points. Two-photon image of LDL (red) colocalization with immunolabeled proteoglycans (green).
(Adapted from Kwon, G. P. et al. 2008.
Circulation
117:2919-2927. With permission from Wolters Kluwer.)
