Biomedical Engineering Reference
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Figure 5.9 Effect of myosin extraction on SHG from myofibrils. Panels a and b show myofibril SHG images
before and after myosin extraction, respectively. Panels c and d show two-photon excitation images of actin labeled
with rhodamine-phalloidin before and after myosin extraction, respectively. Panels e and f show a superposition of
SHG image (in red) and TPE image (in green) before and after the extraction. The size of the images is 20 × 20 μm 2 .
(Modified from Vanzi, F. et al . 2006. J Muscle Res Cell Motil 27 , 469-479. With permission.)
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