Biomedical Engineering Reference
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Figure 5.12 SHG from microtubules. (a) SHG in RBL cells. SHG (green) arises from mitotic spindles (orange
arrows) and from interphase microtubule ensembles (blue arrow). Scale bar: 10 μm. (Modified from Dombeck, D.A.
et al. 2003. Proc Natl Acad Sci 100 , 7081-7086.)
(a)
4
Refractive
index
3.5
1.34
3
1.40
2.5
1.51
2
1.34-ave
1.5
1.40-ave
1
1.51-ave
0.5
0
0
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100 110
NFP (µm)
(b)
3.5
Refractive
index
3
1.34
2.5
1.40
2
1.51
1.5
1.34-ave
1
1.40-ave
0.5
1.51-ave
0
0
10
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NFP (µm)
Figure 8.11 Axial resolution as a function of depth into kidney tissue measured from two-photon fluorescence
of microspheres collected with (a) Olympus 60× NA 1.2 water immersion objective and (b) Olympus 60× NA 1.4
oil immersion ( n = 1.515) objective. The samples were cleared with agents of different refractive indices as shown
in the right panels. (Young, P. A. et al. The effects of spherical aberration on multiphoton fluorescence excitation
microscopy. 2011. J. Microsc. 242: 157-165. Copyright Wiley-VCH Verlag GmbH & Co. KGaA. Reproduced with
permission.)
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