Biomedical Engineering Reference
In-Depth Information
time, both to ensure proper adhesion of MSCs to the scaffold and to
growMSCstotheoptimalnumberswithinascaffold.ToensureMSC
adhesion and expansion, MSC-loaded scaffolds can be incubated for
anywhere from an hour to several days under conditions favorable
forMSCgrowth.Intwo-dimensional(2D)cultureconditions,growth
factors, alone or in combination with dexamethasone, dramatically
enhance proliferation of MSCs. In a three-dimensional (3D) porous
ceramic scaffold, the combination of FGF-2 and dexamethasone
also significantly stimulates both MSC proliferation and osteoblast
lineage commitment. Seeding of bone marrow MSCs in a porous
biphasic calcium phosphate (BCP) scaffold, which is composed of
hydroxyapatite and
β
-tricalcium phosphate (6:4), followed by incu-
bation with FGF-2 and dexamethasone for 7 or 14 days, results in
enhanced growth of MSCs compared to control (Fig. 41.1). Under
Figure 41.1.
GrowthofMSCsinaporousBCPscaffold.BonemarrowMSCs
(2
10
5
cells in a 50
2
mm) by a static method and treated with or without FGF-2 (1 ng/mL), Dex
(10
-
8
M), or a combination of FGF-2 and Dex for 7 and 14 days. Cell num-
bers were measured using a hematocytometer.
Abbreviation
: Dex, dexam-
ethasone. See also Color Insert.
×
μ
L medium) were seeded in BCP ceramics (5
×
5
×
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