PGA FIBER FOR SOFT TISSUE ENGINEERING - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 687

Figure 34.3. Histological finding of formed tissue at 26 weeks. H&E
staining shows histological structures of fibroblast-engineered tendons (a),
tenocyte-engineered tendons (b), a control tissue in control group 2 (c), (d)
and normal pig skin. Collagen III (delicate collagen fibers with a light-green
color) is detected only in the polarized images of control tissues (g and k)
and in normal pig skin (h), as indicated by white arrows. In addition, col-
lagen I (golden color, indicated by white, dotted arrows) is also detected
in these tissues. In the polarized images of (e and i) fibroblast- and (f and
j) tenocyte-engineered tendons and natural tendons (l), collagen I (golden
color) is the predominant collagen type. Original magnification × 400 (i-k);
× 200,allothers.(ReprintedbypermissionfromRef.12). Abbreviation :H&E,
hematoxylin and eosin. See also Color Insert.
was observed among dermal fibroblast- and tenocyte-engineered
tendons and native tendon tissues (Fig. 34.3). Importantly, a strong
mechanical property was also achieved in engineered tendons with
dermal fibroblasts. 12
These two experiments showed the feasibility of using unwo-
ven PGA fibers as a scaffold for in vivo tendon engineering, and
the scaffold did fit the requirement of good biocompatibility,