Cell Migration on Engineered Microstructured Surfaces - Innovative Approaches to Cell Biomechanics - page 128

observed with a L-W1.5 groove in Fig. 9.3d , indicating that a similar mechanism
is inherent in the turning behaviors of cells at the boundary with narrow grooved
surfaces. As shown in Fig. 9.7 , the majority of cells exhibit a turn-type response to
IS-W1.5 grooves.

9.4.2.1 Cell Repelling Effect

Figure 9.7 shows that over 90 % of cells exhibits a turn-type response to IS-W1.5
grooves. The turn-type response at the boundary with the IS-W1.5 grooves
(Fig. 9.6b ) is similar to the response observed with a L-W1.5 groove shown in
Fig. 9.3d . These results indicate that IS-W1.5 grooves and the L-W1.5 groove can
both serve as an effective cell repellent in a similar manner.

9.4.2.2 Cell Trapping Effect

In the stay-type response shown in Fig. 9.6a , pausing and limited protrusion signifi -
cantly decrease cell migration effi ciency. From the viewpoint of groove function,
this behavior can be interpreted as the cell trapping effect.

9.4.2.3 Cell Filtering Effect

For IS-W4 grooves, whether a cell is repelled, or guided and trapped depends on the
angle of approach at the boundary between the fl at and the grooved surfaces. Thus
the grooved surface acts like a motility-based fi lter capable of repelling or trapping
a cell. The details on the cell fi ltering function will be explained in Chap. 10 .

9.5 Fundamental for Manipulation of Cell Migration

by Engineered Micro-environments

Finally in this chapter, we deduce the fundamentals of controlling cell migration by
engineered micro-environments, based on the results of transient changes in migra-
tory behavior of fi sh keratocytes encountering the grooved surface described in
Sects. 9.3 and 9.4 .
Figure 9.8 summarizes the effect of grooved structures on cell migration. By
basic examination as outlined in Sect. 9.3 , we can characterize the behavior of cells
encountering a single line groove and show that a narrow L-W1.5 groove works as