Cell Migration on Engineered Microstructured Surfaces - Innovative Approaches to Cell Biomechanics - page 127

Fig. 9.6 Responses of keratocytes migrating from a fl at surface to an intersecting grooved surface
(Miyoshi et al. 2010 ). All these experiments were done at room temperature. ( a ) Time sequence of
cells exhibiting stay-type response. For fl uorescent microscopy, disaggregated keratocytes were
incubated at 33 °C with Vybrant DiI cell-labeling solution (Invitrogen), diluted 1:100 in the cell
culture medium. After 15 min, the cells were washed three times with the cell culture medium.
Stained cells were imaged every 30 s using a 40×, 0.95NA Plan-Apochromat objective and a
fl uorescent microscope equipped with a CCD camera. The white arrow indicates a cell coming
from the fl at surface to the grooved surface (0-120 s) and being trapped by the grooved surface
(180-540 s). The white arrowhead indicates a cell anchoring to the grooved surface (0-540 s). Red
pseudo color indicates the image focused on the fl at surface. Blue pseudo color in the boxed area
indicates the image focused inside the grooves (on the plane 6 µm deeper than the fl at surface). ( b )
Representative time sequence of turn-type response of a cell. Numbers indicate time in seconds.
The scale bars correspond to 20 µm (Adapted with permission from Elsevier Ltd.: [Biomaterials],
copyright (2010))
Fig. 9.7 Frequency of
keratocyte responses to the
intersecting grooves (IS-W4,
IS-W1.5) (Miyoshi et al.
2010 ). The number of cells is
17 for IS-W4, and 16 for
L-W1.5 (Adapted with
permission from Elsevier
Ltd.: [Biomaterials],
copyright (2010))