Biomedical Engineering Reference
In-Depth Information
(A)
20
15
10
5
0
(B)
80
70
60
50
40
30
20
10
0
60,000
50,000
40,000
30,000
20,000
10,000
0
-13
-12 -11 -10
Log
10
[lmmTAC-NYESO] (M)
-9
-8
-13
-12 -11 -10
Log
10
[lmmTAC-NYESO] (M)
-9
-8
FIGURE 32.5
Targeting NY-ESO-1 presenting cancer cells with ImmTAC. (A) NY-ESO-1
peptide: MHC epitopes on the surface of cancer cells were determined using the high-affinity
NY-ESO-1 mTCR (see Table 32.2). NY-ESO-1-positive IM9 cells were stained with biotinylated
high-affinity NY-ESO mTCR and streptavidin-PE, and the individual epitopes observed as individual
spots (middle panel), were counted using phase-contrast microscopy. The graph shows that the
number of NY-ESO-1 epitopes presented were in the range 2-13 per cell. A high-affinity TCR
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