Biomedical Engineering Reference
In-Depth Information
pEGFP-C1
pEGFP-C1
Complexation
G2-PAMAN
G2-MSN
G2-PAMAM
Endocytosis
Nucleus
pEGFP-C1
G2-PAMAN
Green fluorescent proteins
(a)
(b)
FIGURE 8.8
Schematic representation of a nonviral gene transfection system based on a Texas Red-loaded,
G2-PAMAM dendrimer-capped MCM-41 type mesoporous silica nanospheres (MSNs) loaded with an
enhanced green fl uorescent protein (GFP) plasmid DNA (pEGFP-C1). Fluorescence confocal micrographs
of cells transfected by the pEGFP-C1-coated G2-MSN system are shown below. Picture (a) presents GFP-
transfected HeLa cells showing cross-sections through a cell layer. Orthogonal images indicated the mono-
layer packing of cells. Picture (b) presents Texas Red-loaded G2-MSNs inside a GFP-transfected rat neural
glia cell. (Reprinted from Radu, D.R. et al.,
J. Am. Chem. Soc.
, 126, 13216, 2004.)
