Biomedical Engineering Reference
In-Depth Information
d
n
4
y
3
n
g
|
7
Figure 9.9
Illustrative preparation of PTX and QD-loaded micelle and pH-tunable
drug release.
14
first self-assembled into core-shell micelles with DOX loaded in the core. Then
BCL-2 siRNA was incorporated into the micelles by complexation with the
cationic shell. Finally, driven by the electrostatic interaction, folic acid-
functionalized poly(ethylene glycol)-block-poly(glutamic acid) (FA-PEG-b-
PGA) was coated onto the surface of the cationic PEI-PCL nanoparticles pre-
loaded with siRNA and DOX, potentiating a ligand-directed delivery to the
human hepatic cancer cells Bel-7402. The multifunctional hierarchical nano-
assembly enabled simultaneously delivering siRNA and drug into the same
cancer cells, yielding a synergistic effect of RNA interference and chemother-
apy in cancer. At optimized composition, the nanoparticles exhibited not only
high
transfection
efficiency
but
also
ideally
controlled
release
of
drug.
Compared
to
nonspecific
delivery,
the
folate-targeted
delivery
of
BCL-2
