Biomedical Engineering Reference
In-Depth Information
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Figure 8.6
Silencing TNF-a secretion determined with ELISA due to AS-ODN
administration to LPS-stimulated 48 h pre-cultured thio-Mwsfor
different dose conditions. (A) AS-ODN dose dependence of the silencing,
after incubation for 30 min at 37 uC. (B) dA tail length dependence of the
silencing for the complexed (c) and naked (n) AS-ODN (ASODN; 0.5
mM); *P , 0.01 versus the cells in PBS. (C) Comparison of AS-ODN/
SPG, sense-ODN/SPG, and scrambled-ODN/SPG (AS-ODN, 0.5 mM);
N.D. 5 not detected. Data represent the mean ยก SD of triplicate wells.
